30 Rock features incidental LGBTQ+ representation, primarily through Jenna Maroney's consistent bisexuality, which is accepted within the show's comedic framework. While some jokes may touch on stereotypes or ignorance, these are often satirized or countered by other characters, resulting in a net neutral impact where positive and negative elements largely balance out.

30 Rock features a transsexual character, Beverly, in the episode 'Christmas Attack Zone.' Her identity is primarily used as a comedic plot device to generate humor from Jack Donaghy's discomfort and the shock of her transition. The portrayal lacks depth and dignity, framing trans identity as a source of awkwardness rather than affirming it, leading to a net negative impact.

While "30 Rock" frequently satirizes the cultural aspects, hypocrisy, or literal interpretations of Christianity through characters like Jack and Kenneth, it ultimately portrays genuine faith (e.g., Kenneth's unwavering goodness) with an underlying warmth and respect. The humor is often directed at the absurdity of human behavior or societal norms, not the core tenets of the faith itself.

Judaism is primarily depicted through cultural references and character backgrounds (e.g., Liz Lemon's implied heritage, New York demographics). The show employs observational and self-deprecating humor related to Jewish stereotypes, but these are generally lighthearted and contribute to character identity rather than presenting the religion or its adherents as problematic.