9-1-1 offers a strong positive portrayal of LGBTQ+ characters, primarily through Hen Wilson and her wife Karen. Their relationship is a central, stable, and loving anchor, depicted with dignity and complexity. The show consistently normalizes queer families and relationships, affirming the worth of LGBTQ+ lives and love by framing obstacles as external rather than inherent to their identity.

The show '9-1-1' includes transsexual characters whose identities are depicted with dignity and respect. While they may face external challenges such as hate crimes or medical issues, the narrative consistently affirms their worth and portrays their lives with empathy, avoiding harmful stereotypes.

The show features Maddie Buckley, who engages in a desperate close-quarters physical struggle against her abusive ex-husband. She successfully defends herself and incapacitates him using a knife, securing her victory in the confrontation.

The show consistently portrays Christianity, particularly through Bobby Nash's character, as a source of strength, moral guidance, and redemption. His faith is depicted with respect and nuance, showing its positive impact on his life and his ability to help others.