Batwoman features openly lesbian lead characters, Kate Kane and Ryan Wilder, whose identities and relationships are central to the narrative. The show consistently portrays LGBTQ+ characters with dignity, complexity, and agency, treating their romantic relationships with respect and depth. Obstacles faced are external, affirming the worth of queer lives and love within a superhero context.

Batwoman features Nia Nal (Dreamer), a trans woman superhero, portrayed with dignity, agency, and complexity. Her trans identity is acknowledged but not a source of mockery or villainy. The narrative consistently presents her in a positive and affirming light, focusing on her heroism and personal growth.

The show features multiple female characters, including Kate Kane and Ryan Wilder as Batwoman, and Alice, who consistently engage in and win close-quarters physical fights against male opponents using martial arts and hand-to-hand combat.

The show features characters such as Sophie Moore and Mary Hamilton, who were originally depicted as white in the DC Comics source material, but are portrayed by actresses of different races in the 2019 series.

The series portrays Judaism as an integral and respected part of protagonist Kate Kane's identity and family heritage. It is depicted through cultural practices like Hanukkah celebrations and symbols, affirming its presence without critique.