The 2016 Ben 10 animated series does not feature any explicit or identifiable LGBTQ+ characters or themes. The narrative focuses on Ben Tennyson's adventures without incorporating elements related to queer identity or relationships, resulting in no discernible LGBTQ+ portrayal.

The 'Ben 10' animated series, across its various iterations, does not include any explicit or identifiable transsexual characters or themes. The narrative focuses on sci-fi action and alien transformations, with no depiction of transgender identity.

The show primarily features Gwen Tennyson as a significant female character who participates in combat. However, her victories against male opponents are predominantly achieved through the use of her Anodite (mana) powers, which involve energy projection, shields, and telekinetic manipulation, rather than direct close-quarters physical combat or martial arts.

The 2016 "Ben 10" reboot maintains the established genders of its core characters, such as Ben Tennyson, Gwen Tennyson, and Grandpa Max, from previous iterations of the franchise. No significant characters underwent a gender change.

The 2016 animated series "Ben 10" is a reboot of the original 2005 series. The core characters, including Ben, Gwen, and Grandpa Max, maintain the same racial depictions as their original counterparts, with no instances of a character established as one race being portrayed as a different race.