Bravest Warriors offers a positive portrayal of LGBTQ+ characters and themes. Chris Kirkman's bisexuality is depicted naturally, and the romantic relationship between Plum and Beth is shown as loving and stable. The series generally normalizes diverse identities, presenting queer characters and relationships without ridicule or sensationalism, contributing to an inclusive and affirming narrative.

Bravest Warriors features Plum, a significant recurring character who explicitly identifies as genderfluid. Her identity is revealed in a matter-of-fact manner and is met with immediate, unconditional acceptance by the protagonist, Chris. This portrayal is affirming, presenting Plum's gender identity as a natural and respected aspect of her complex character, free from mockery or negative stereotypes, contributing positively to the show's inclusive themes.

The show features Beth Tezuka, a main female character, who frequently participates in close-quarters combat. She is shown using melee weapons and physical attacks against various male-coded opponents, contributing to their defeat.