Brickleberry's portrayal of LGBTQ+ themes is overwhelmingly negative. The show consistently uses homophobic slurs, stereotypes, and non-heteronormative identities as a source of crude, offensive humor. Depictions are primarily for mockery and shock value, lacking dignity, complexity, or any affirming counterbalance. The narrative reinforces harmful stereotypes without critique.

Brickleberry features a character, Denzel, who transitions to Denzella, with the portrayal being largely negative. The show uses the character's gender identity as a source of crude humor and shock value, relying on stereotypes and mockery. There are no affirming or validating elements for transsexual themes, making the net impact problematic.

The show consistently portrays Christianity, its practices, and adherents in a highly satirical and negative light, often highlighting hypocrisy, ignorance, or extreme fundamentalism for comedic effect without offering counterbalancing nuance.

Judaism is depicted through irreverent humor and stereotypes, often for shock value, reinforcing negative portrayals without any positive or nuanced counterpoints.