Call the Midwife offers a largely positive portrayal of LGBTQ+ characters, depicting them with dignity and empathy despite the era's harsh realities. The show consistently critiques societal prejudice and legal persecution as external forces, affirming the worth of queer lives and love through compassionate storytelling.

Call the Midwife features a transsexual character, Mrs. Aylward (Colette), whose story is handled with dignity and empathy. The narrative explores her journey of living authentically, highlighting the challenges she faces due to societal prejudice while portraying the midwives and doctors as supportive and affirming of her identity. The show's overall stance is respectful and validating.

The series consistently portrays Christianity, particularly through the Anglican nuns and nurses, as a source of profound compassion, unwavering dedication to service, and moral guidance. It explores the complexities of faith, doubt, and resilience, ultimately affirming its positive impact on individuals and the community.

The show depicts Jewish characters and their traditions with respect and cultural sensitivity, highlighting the strength of their community, family bonds, and religious practices. It often portrays their resilience and the importance of their faith in navigating life's challenges.