Childrens Hospital features incidental LGBTQ+ elements, primarily through Chief's fluid sexuality and the homoerotic subtext between Owen and Glenn. These aspects are consistently played for absurdist comedic effect within the show's satirical framework. While present, they are not central to character development or narrative themes, and neither affirm nor denigrate queer identity in a meaningful way, resulting in a neutral overall portrayal.

Childrens Hospital features a brief depiction of a patient requesting a sex change operation. This is handled with the show's characteristic absurd humor, where a clown doctor performs the procedure incompetently. The portrayal trivializes the medical aspect of gender transition, using it as a comedic plot device without exploring the character's identity with dignity or respect, resulting in a problematic net impact.

The show consistently portrays characters associated with Christian beliefs as deeply hypocritical, self-serving, or absurd. The narrative uses satire to highlight the perceived foolishness and moral failings of these adherents, offering no counterbalancing positive or nuanced perspective on the faith itself.