"Conan" consistently presented a positive portrayal of LGBTQ+ individuals and themes. The show's host, Conan O'Brien, often voiced support for LGBTQ+ rights, and the program regularly featured respectful interviews with LGBTQ+ celebrities. While a comedy show, its humor generally avoided harmful stereotypes, contributing to an affirming and inclusive environment.

As a long-running late-night talk show, 'Conan' occasionally included references to or discussions of transsexual themes and identities, primarily through news segments, guest interviews, or comedic sketches. These depictions were generally incidental, lacking a consistent positive or negative narrative arc, and thus did not establish a definitive stance on transsexual identity.