Daredevil: Born Again features multiple transgender characters in significant, integrated roles within Matt Murdock's legal cases. Their portrayal is substantive, contributing meaningfully to the plot rather than being tokenized. The show appears to approach LGBTQ+ representation with nuance, ensuring queer identity is part of a character's whole, indicating a respectful and affirming depiction.

Jack Duquesne, a character traditionally depicted as a white American in the source material, is portrayed by a Mexican-American actor. Additionally, Vanessa Fisk, typically a white woman of European descent, is played by an Israeli actress with Middle Eastern features. These casting choices represent a change in race from their established comic book depictions.

Daredevil: Born Again" portrays Catholicism as a central, deeply explored aspect of Matt Murdock's identity. The narrative respectfully delves into his spiritual struggles, themes of faith, redemption, and the tension between his beliefs and vigilante life, using Catholic symbolism to add depth.