The animated film "Dark Knight, 2000" does not include any discernible LGBTQ+ characters or themes. Its narrative focuses on traditional Arthurian legend elements without exploring queer identities or experiences, resulting in no portrayal to evaluate.

The film 'Dark Knight, 2000' does not feature any identifiable transgender characters or themes. Consequently, there is no portrayal to assess regarding its impact on transsexual representation. For example, no character arcs or plot points related to transgender identity are present in the provided information.

The film 'Dark Knight' (2000) primarily focuses on the male protagonist's quest and combat encounters. There are no depicted scenes where a female character engages in and wins close-quarters physical combat against one or more male opponents.

The film "Dark Knight" (2000) is an original fantasy production, not an adaptation of existing source material or a reboot of a franchise with pre-established characters. There is no evidence of characters being portrayed with a different gender than their canonical or historical baseline.

The film 'Dark Knight, 2000' is an adaptation of the Ivanhoe stories. Key characters like Ivanhoe and Rebecca are canonically white, and the actors portraying them are also white, indicating no race swaps based on the provided cast and source material.