Dash & Lily features multiple LGBTQ+ characters, including Lily's brother Langston and Dash's best friend Boomer. Their gay identities and relationships are portrayed positively, with dignity, and integrated naturally into the narrative. The show affirms the worth of their lives and love without making their sexuality a source of mockery or undue conflict.

Lily, a character generally understood to be white in the source novel series "Dash & Lily's Book of Dares," is portrayed by Midori Francis, an actress of mixed race (Japanese-American). This constitutes a race swap for a main character.

The film portrays the cultural celebration of Christmas, a holiday rooted in Christianity, with warmth and positivity, emphasizing themes of connection, family, and joy. While not overtly religious, the narrative frames the holiday spirit as something valuable and affirming.

Judaism is depicted positively through the celebration of Hanukkah by Boomer's family, showcasing its traditions, warmth, and importance within the family unit. The portrayal is respectful and highlights the shared values of holiday celebrations.