Elfen Lied does not feature any identifiable LGBTQ+ characters or themes within its main plot or character arcs. The series' focus is on sci-fi horror, violence, and the complex, often tragic, relationships between its predominantly heterosexual characters, particularly Kouta and the Diclonius girls.

The anime series "Elfen Lied" does not feature any identifiable transsexual characters or themes. Its narrative focuses on genetic mutation, discrimination, trauma, and the nature of humanity, without exploring gender identity in the context of transsexuality.

Female characters in the show, such as Lucy, Nana, and Mariko, engage in combat and defeat male opponents. However, their victories are achieved exclusively through the use of their Diclonius vectors, which are powerful telekinetic superpowers, rather than through physical skill, strength, or martial arts.

The anime adaptation of "Elfen Lied" maintains the established genders of all significant characters from its source manga. No characters originally depicted as one gender in the manga are portrayed as a different gender in the anime.

Elfen Lied is an anime adaptation of a Japanese manga. All characters in the anime are portrayed consistently with their visual depictions in the original manga, with no changes to their implied or depicted race.