The show "FBI" includes LGBTQ+ characters, such as Maggie Bell's sister, Erin, whose sexuality is depicted as a normal aspect of her life. While not central to the series' main narratives, these portrayals are generally respectful and avoid harmful stereotypes, neither strongly affirming nor denigrating LGBTQ+ identity.

The series features a transgender character, Sarah, in a single episode. Her identity is portrayed with dignity and empathy, with the narrative framing external prejudice and her tragic death as external forces. FBI agents treat her respectfully, affirming the worth of her life by diligently investigating her murder. The overall portrayal is supportive and validating.

Female FBI agents Maggie Bell and Tiffany Wallace are frequently depicted engaging in and winning close-quarters physical confrontations against male suspects during arrests, utilizing their training to subdue opponents.

The show often features characters with Christian backgrounds portrayed positively. When Christian extremism is depicted, the narrative clearly condemns the extremist actions and ideology, distinguishing it from mainstream Christianity and positioning the audience against the perversion of faith.

The series often features Muslim characters portrayed positively or neutrally. While episodes may involve Islamic extremism, the narrative consistently distinguishes between the faith and radicalized individuals, condemning terrorism and bigotry while affirming the dignity of mainstream Islam.

Judaism is typically portrayed with respect, often through characters whose faith is a positive aspect of their identity. When antisemitism or crimes against Jewish communities are depicted, the narrative unequivocally condemns such bigotry, positioning the audience to sympathize with the victims.