Friends presented a mixed portrayal of LGBTQ+ characters and themes. The show featured a groundbreaking lesbian couple, Carol and Susan, whose relationship was depicted with dignity and love. However, other instances, such as the portrayal of Chandler's gay cross-dressing father and some dated jokes, relied on stereotypes or discomfort for humor, resulting in a net neutral impact.

Friends features Charles Bing (Helena Handbasket), Chandler's father, a drag queen who identifies as a woman. The portrayal often uses Charles's gender expression and identity as a source of comedic discomfort for Chandler, relying on stereotypes and conflating drag with trans identity. While there's eventual acceptance, the narrative largely lacks a critical perspective on the problematic humor, resulting in a net negative impact.

The show portrays Judaism as a respected cultural heritage, primarily through the Geller family. Ross's efforts to teach his son about Hanukkah are depicted with warmth and humor, affirming the importance of cultural identity without critiquing the faith itself.