General Hospital has consistently featured LGBTQ+ characters and storylines, generally portraying them with dignity and complexity. Characters like Lucas Jones, Kristina Davis, and Dr. Terry Randolph have had significant arcs where their identities were affirmed and integrated positively into the show's narrative, despite typical soap opera drama.

General Hospital featured Dr. Terry Randolph, a trans woman physician, who was portrayed with dignity and professionalism. Her character was integrated respectfully into the community, engaging in storylines beyond her gender identity, reflecting an affirming and validating depiction of a transsexual individual.

The show frequently features characters who identify as Christian, attend church, and grapple with moral dilemmas through a faith-based lens. While individual characters may be flawed or hypocritical, the narrative generally presents Christian faith as a source of comfort, guidance, and community, distinguishing between personal failings and the virtues of the religion itself.