Girls features Elijah Krantz as a significant gay character whose identity is integrated into the narrative without being the primary source of conflict or mockery. While his portrayal offers complexity and agency, it occasionally leans into stereotypes, consistent with the show's satirical depiction of all its flawed characters. The series neither strongly affirms nor denigrates LGBTQ+ identity, presenting it as a natural part of its characters' lives.

The show portrays several main characters as culturally Jewish, exploring their heritage, family dynamics, and identity with nuance and depth. While humor and satire are sometimes used around cultural aspects, the narrative does not condemn Judaism itself, but rather treats it as a complex and integral part of the characters' backgrounds.