Grey's Anatomy consistently features a diverse array of LGBTQ+ characters, including prominent lesbian, bisexual, and gay relationships, as well as transgender representation. The show generally depicts these characters with depth, agency, and dignity, exploring their personal and professional lives, often using their stories to promote understanding and acceptance. The net portrayal is overwhelmingly positive and affirming.

Grey's Anatomy features Dr. Casey Parker, a trans man, as a recurring character, portraying him with dignity, competence, and agency. The show handles his trans identity and related medical issues with respect and empathy, framing obstacles as external and affirming the worth of trans lives within the narrative. Overall, the portrayal is supportive and validating.

The show frequently portrays Christian characters, like Miranda Bailey, whose faith is depicted as a significant aspect of their identity, providing moral guidance and comfort, even when challenged by life's difficulties. The narrative generally treats these beliefs with respect and nuance.

When Muslim characters appear, typically as patients or guest stars, their faith is handled with sensitivity and respect. The narrative focuses on their individual stories and medical needs, avoiding problematic generalizations.

Jewish characters and cultural traditions are occasionally featured, often as part of a character's background or family life. These portrayals are consistently respectful, integrating their faith or heritage without negative stereotyping.