Harvey Girls Forever! features Frufru, an openly gay character whose crush on another girl is depicted as a normal part of childhood experiences. The show portrays her identity positively and with dignity, avoiding stereotypes or making her orientation a source of conflict. This affirming representation normalizes LGBTQ+ identities for its target audience.

The show features Lotta, a female character with immense physical strength. She frequently demonstrates her superior power by effortlessly overpowering male characters, such as the Bloogey Boys, in various physical contests and comedic brawls.

Dot, one of the main characters, was originally depicted as a white character in the Harvey Comics source material. In 'Harvey Girls Forever!', she is portrayed as a Black character, which constitutes a race swap.