How I Met Your Mother features a significant positive portrayal through James Stinson, Barney's openly gay brother, who is depicted as a loving husband and father. While some early jokes were problematic, the show ultimately affirms James's identity and family, presenting them with dignity and integrating them into the core narrative without judgment.

How I Met Your Mother includes several problematic jokes, primarily from Barney, that use trans identity for comedic effect. These instances often rely on slurs and stereotypes, framing trans identity as a source of surprise or discomfort. The show lacks any affirming or complex portrayals, resulting in a net negative impact.

The show portrays Christianity as a warm cultural background for characters like Marshall and his family, often associated with tradition, community, and sentimental moments, without being depicted as oppressive or foolish.

James's conversion to Judaism and his family's practices are depicted with respect and warmth, showcasing the adoption of new traditions and a loving family environment.