The animated series "Johnny Test" does not include any identifiable LGBTQ+ characters or themes. Its narrative centers on the titular character's adventures with his super-genius sisters, without exploring queer identities or relationships.

The animated series "Johnny Test" does not feature any identifiable transsexual characters or themes throughout its episodes. Consequently, there is no portrayal to evaluate as positive, negative, or neutral.

The show primarily features comedic, invention-based conflicts. While female characters are present and participate in various scenarios, there are no instances where they are depicted as physically defeating one or more male opponents in close-quarters combat through skill, strength, or martial arts.

The 2021 reboot of Johnny Test features the same established characters as the original series, and all retain their canonical genders. No characters previously established as one gender are portrayed as a different gender.

The 2021 revival of "Johnny Test" features the same core characters who retain their established racial appearances from the original animated series. No characters canonically established as one race in prior installments are portrayed as a different race.