King of the Hill features several LGBTQ+ characters and themes, most notably the coming out and marriage of Dale Gribble's father. While humor is often derived from the main characters' reactions, the show ultimately portrays these relationships and identities with dignity and acceptance, affirming their worth despite initial comedic obstacles. The net impact is positive.

The series includes a storyline where a character's gender presentation is used as a comedic plot device, leading to shock and embarrassment. This depiction frames gender non-conformity as a source of mockery rather than exploring identity with dignity or complexity, contributing to a negative overall portrayal.

The show frequently satirizes the hypocrisy and narrow-mindedness of individual Christian characters, particularly Hank and Peggy. However, the narrative consistently affirms the positive aspects of faith, community, and moral guidance derived from Christianity, often portraying the church as a vital institution and a source of comfort. The satire targets human flaws within the faith, not the faith itself.