Last Week Tonight with John Oliver consistently portrays LGBTQ+ themes and individuals in a positive and affirming light. The show actively advocates for LGBTQ+ rights, critiques discriminatory practices, and frames prejudice as an external societal problem, always maintaining a respectful and empathetic stance.

Last Week Tonight with John Oliver consistently covers transgender issues, dedicating segments to critique anti-trans legislation and misinformation. The show's portrayal is affirming, advocating for trans rights and depicting trans individuals with empathy and respect. It frames obstacles as external prejudice, promoting understanding and validating trans identities through factual reporting and satire.

The show consistently critiques the problematic actions and rhetoric of various Christian institutions and figures, particularly televangelists, anti-LGBTQ+ groups, and those using faith for political gain or exploitation. The narrative frames these as serious issues, highlighting hypocrisy and abuse of power within these public manifestations of the religion.

The show frequently highlights and condemns Islamophobia, the misrepresentation of Muslim communities, and the bigotry faced by adherents. While it critiques extremist groups or problematic governments, its narrative consistently positions the audience to sympathize with the religion's adherents against prejudice, aligning with a positive portrayal of the faith itself.