Late Night with Seth Meyers consistently features LGBTQ+ guests and discusses related issues with a respectful, empathetic, and affirming tone. The show actively advocates for LGBTQ+ rights and critiques prejudice, portraying queer identity with dignity and complexity, leading to a net positive impact.

Late Night with Seth Meyers consistently offers a platform for transgender voices, featuring trans celebrities and activists who share their experiences with dignity. Its topical segments often address trans rights and issues with empathy, contributing to a net positive and affirming portrayal of transsexual characters and themes.

The show frequently satirizes the hypocrisy, judgmentalism, or harmful political actions of Christian politicians and the Christian right, portraying these adherents and institutions in a critical light without significant counterbalancing positive portrayals within those satirical contexts.

When Islam is discussed, the show's narrative often critiques Islamophobia and bigotry against Muslims, positioning the audience to sympathize with the religion and its adherents against prejudice.

While political issues related to Judaism may be discussed, the show's narrative consistently condemns antisemitism and bigotry against Jewish people, positioning the audience to sympathize with the community when it faces prejudice.