Little Britain features prominent LGBTQ+ characters whose portrayals largely rely on exaggerated stereotypes and problematic humor. The show often derives laughs from their identities being a source of ridicule or misgendering, lacking dignity or affirmation. This contributes to a net negative impact on the depiction of LGBTQ+ themes.

Little Britain features Emily Howard, a character whose identity as a woman is consistently mocked through exaggerated stereotypes and the 'man in a dress' trope. The portrayal relies on ridicule, presenting her identity as a source of humor without dignity or affirmation, contributing to a negative overall impact.

The show frequently features vicars as recurring characters, often portraying them as naive, easily flustered, or out of touch with modern sensibilities. While the satire is broad and targets various aspects of British society, the depiction of these religious figures contributes to a comedic stereotype of the institution as somewhat foolish or ineffectual, without offering counterbalancing positive portrayals.