Magic Knight Rayearth does not feature any identifiable transsexual characters or themes within its narrative. The story primarily focuses on three middle school girls summoned to a magical world, with no plot points or character arcs exploring transgender identity. Consequently, there is no portrayal to evaluate regarding transsexual characters or themes.

The primary female characters, Hikaru, Umi, and Fu, engage in combat using magical swords and piloting giant magical robots (Rune-Gods). Their victories against male opponents are consistently achieved through the use of powerful magic spells and their mecha, rather than through non-superpowered physical skill, strength, or martial arts.

Magic Knight Rayearth (1994) is an anime adaptation of the manga series by CLAMP. The anime faithfully portrays the genders of its main characters as established in the original source material, with no instances of a character's gender being changed.

The 1994 anime adaptation of Magic Knight Rayearth portrays its main characters, Hikaru, Umi, and Fuu, as Japanese, consistent with their origin in the Japanese manga. The race of the English dub voice actors does not alter the visual depiction of the animated characters.