@midnight with Chris Hardwick featured incidental mentions of LGBTQ+ topics as part of its internet-focused comedy. These brief references were neither strongly positive nor negative, treating queer identity as another element of pop culture for comedic purposes, resulting in a neutral overall portrayal.

The show frequently features jokes targeting internet content related to conservative Christian viewpoints, religious figures, or instances of perceived hypocrisy within Christian communities. The humor often highlights perceived foolishness or absurdity, and the show's comedic framing validates these critiques without offering counterbalancing nuance or sympathetic portrayals of the faith.

Judaism is often depicted through comedic references to its holidays and cultural traditions, sometimes playing on stereotypes. The show's satirical approach, even when lighthearted or self-deprecating, tends to emphasize perceived eccentricities for laughs, reinforcing certain cultural stereotypes without affirming the faith's dignity.