MSNBC Prime consistently features LGBTQ+ characters and themes through news segments and guest interviews. The program's overall approach is supportive, highlighting LGBTQ+ rights, challenges, and achievements with an empathetic and affirming tone, presenting queer identity with dignity and respect.

The show's narrative frequently critiques the political influence and social stances of conservative Christian groups, often portraying their actions or doctrines as hypocritical, oppressive, or harmful within the context of contemporary issues.

The show consistently condemns Islamophobia and often features Muslim voices, portraying Muslim communities and their concerns with respect and sympathy, particularly when addressing issues of bigotry or social justice.

The show consistently condemns antisemitism and often features Jewish voices, portraying Jewish communities and their concerns with respect and sympathy, particularly when addressing issues of bigotry or social justice.