The 'Night Court' revival features Olivia Moore, a main character who is openly lesbian. Her identity is normalized and depicted with dignity, complexity, and agency. The show integrates her relationships seamlessly, presenting them without mockery or negative stereotypes, contributing to a net positive and affirming portrayal of LGBTQ+ characters.

Night Court featured transsexual characters in multiple episodes during the 1980s. While initial reactions from some characters included discomfort, the show consistently resolved these storylines with empathy and acceptance. Narratives challenged prejudice and affirmed the dignity and rights of transsexual individuals, particularly through Judge Harry Stone's evolving understanding.

The show frequently features characters who are nominally Christian or operate within a Christian-influenced cultural context. While individual adherents may be portrayed comically or as flawed, the narrative generally treats Christian values (like compassion and justice, often embodied by Judge Stone) with respect. The humor typically targets human foibles or absurd situations rather than the core tenets of the faith.

Jewish characters, such as Roz Russell, are depicted as integral parts of the show's diverse cast. Their religious and cultural background is treated with respect, often contributing to their character development or comedic situations without denigrating the faith itself.