ONE PIECE (2023) offers a predominantly positive and affirming portrayal of LGBTQ+ characters and themes. It integrates gender-fluid and trans characters with dignity and respect, whose identities are accepted within the narrative. Queer-coded main characters and subtle queer relationships contribute to diverse representation. The show also respectfully handles stereotypes, emphasizing self-acceptance and pride, weaving queer themes into its broader message of freedom and diversity.

One Piece (2023) features trans and gender-nonconforming characters like Kiku, a trans woman samurai, and Yamato, who identifies as a man without conforming to typical masculine appearances. Characters like Bon Clay also embody gender fluidity. The show integrates these identities respectfully and naturally into the story, aligning with themes of diversity and acceptance without making gender identity their sole focus.

The series features Nami, who participates in a large-scale battle against male fishmen. She uses her staff and tactics to fight effectively, contributing to her group's victory over the male opponents.

The live-action adaptation portrays Sham, a character originally established as male in the source manga and anime, as female. This change constitutes a gender swap according to the defined criteria.

The show incorporates symbolic references, such as Adam and Eve, and themes aligned with Christian values like community, sacrifice, and hope. These elements are used for aesthetic and thematic enrichment, contributing positively to the narrative's depth without direct religious promotion or critique.