P-Valley offers a deeply affirming and nuanced portrayal of LGBTQ+ characters, particularly through Uncle Clifford, a non-binary club owner. The show depicts queer individuals with dignity, complexity, and agency, exploring their relationships and identities within a supportive community. It frames challenges as external, consistently validating the worth of LGBTQ+ lives and love.

P-Valley offers a primarily positive portrayal of trans and gender non-conforming identities through its central character, Uncle Clifford. Depicted with dignity, complexity, and agency, Uncle Clifford's journey as a club owner and lover is explored with respect, affirming the worth of their identity and relationships amidst external challenges.

P-Valley portrays Christianity with significant nuance, showcasing both its role as a source of community, solace, and moral guidance for some characters, and the hypocrisy or judgmentalism of others. The narrative critiques the flawed actions of individuals rather than the faith itself, often positioning the audience to sympathize with characters who find genuine comfort or struggle with their faith within a complex world.