Party Down features LGBTQ+ characters in incidental roles, primarily as guests or clients at catered events. Their identities are acknowledged but not central to the main narrative or character development, resulting in a neutral portrayal that neither strongly affirms nor denigrates.

Party Down features a transgender character, Jackal Onassis, a successful rock star. Her identity is present but not central to the narrative, which focuses on the chaos of her celebrity party. The portrayal avoids strong positive or negative arcs, neither uplifting nor denigrating trans identity, thus resulting in a neutral impact.

The show frequently satirizes conservative Christian characters, often portraying them as hypocritical, judgmental, or naive. The narrative uses these portrayals to critique certain aspects of religious performance and conservative culture, without offering significant counterbalancing positive views of the faith itself.