Pennyworth features LGBTQ+ characters, notably Alfred's father, Arthur Pennyworth, and Lord Harwood. The series portrays their identities with dignity and complexity, exploring the challenges of being gay in 1960s London, such as secrecy and blackmail, as external societal pressures. The narrative maintains an empathetic stance, granting these characters agency and avoiding harmful stereotypes, resulting in a net positive portrayal.

Pennyworth: The Origin of Batman's Butler features Katie Browning, a trans woman scientist, in its third season. Her character is depicted with dignity, intelligence, and agency, playing a significant role in the narrative. Her trans identity is presented matter-of-factly, without being a source of ridicule or conflict, contributing to a positive and affirming portrayal.