Person of Interest features a significant and affirming portrayal of a queer relationship between two main characters, Root and Shaw. Their love story is developed with dignity, complexity, and agency, becoming a vital emotional core of the series. Despite tragic elements, the narrative consistently validates their lives and love, avoiding harmful stereotypes.

Person of Interest features Claire Phipps, a transsexual character whose identity is handled with respect and affirmation. She is depicted as a brilliant hacker, and her gender identity is accepted without question by the protagonists. The narrative frames her challenges as external threats, not related to her identity, ultimately providing a supportive and validating portrayal.

The show features female characters, notably Sameen Shaw and Root, who frequently engage in and win direct physical combat against multiple male opponents. They utilize martial arts and close-quarters fighting techniques to overcome their adversaries.

The series portrays Christian characters, such as Father Michael, as compassionate and morally upright figures who actively work to help others. Biblical references are often used by characters like Finch to underscore themes of justice, sacrifice, and the inherent value of human life, aligning the narrative with virtues often associated with the faith. The show's narrative consistently frames these elements in a respectful and affirming manner.