Regular Show does not feature any identifiable LGBTQ+ characters or themes throughout its run. The narrative primarily focuses on the surreal adventures and heterosexual relationships of its main characters, with no explicit or implicit queer representation present in the series.

Regular Show does not feature any identifiable transsexual characters or explicitly explore related themes. The narrative primarily focuses on the surreal misadventures of its main characters, with no explicit or implicit engagement with transgender identity or experiences.

Regular Show primarily features male protagonists and antagonists. While female characters like Starla exhibit strength and CJ possesses superpowers, there are no clear instances of a female character defeating one or more male opponents in direct physical combat using skill, strength, or martial arts.

Regular Show is an original animated series, and all its characters were created for the show. There is no prior source material, historical figures, or previous installments from which characters' genders could have been established and subsequently changed.

Regular Show is an original animated series, not an adaptation or reboot of pre-existing material. Its characters were created for the show, establishing their races within its own canon. Therefore, no character could have been race-swapped from a prior established version.