The portrayal of LGBTQ+ characters and themes in 'Reno 911!' is predominantly negative. The show frequently uses queer identity as a source of crude humor, relying on stereotypes and the discomfort of its characters. While satirical, the comedic approach often ridicules rather than affirms, presenting little positive counterbalance.

Reno 911! includes occasional minor characters identified as 'transvestites' or gender non-conforming individuals. Their portrayal is primarily comedic, with humor often stemming from the deputies' ignorant reactions, confusion, and the use of outdated terminology. The show's satirical style frequently relies on stereotypes, resulting in a depiction that lacks dignity or complexity and contributes to a problematic overall impact.

The show frequently portrays Christian characters, particularly Deputy Wiegel, as hypocritical, prejudiced, and foolish, often using their faith to justify their bizarre or morally questionable actions. The narrative offers no significant counterbalancing positive portrayal, reinforcing a comedic stereotype of certain Christian adherents.

When Islam is referenced, it is typically through the lens of the officers' ignorance, prejudice, or incompetence. The show's satire is directed at the officers' bigotry and profiling, positioning the audience to recognize and condemn such prejudice, thereby affirming the dignity of the religion against character-driven attacks.