Resident Alien includes a minor lesbian character, Carly, whose identity and relationship are presented as normal and accepted. While this depiction is positive, LGBTQ+ themes are not central to the show's narrative, resulting in a neutral overall impact on its portrayal.

Resident Alien features Sahar, a trans woman, as a recurring character. Her trans identity is acknowledged but not central to the plot, nor is it a source of conflict or mockery. The portrayal is respectful and incidental, integrating her as a normal member of the community without deep exploration of her trans experience.

The show observes Christian practices through an alien's detached, often humorous, perspective. While Harry finds aspects baffling, the narrative generally portrays adherents as good-hearted and highlights the positive community and comfort faith provides, without condemning the religion itself.