Robot Chicken frequently employs LGBTQ+ characters and themes in its satirical sketches, often using their identity as the basis for crude or stereotypical jokes. This approach, while characteristic of the show's irreverent humor, tends to endorse ridicule and reinforce harmful stereotypes without critique, resulting in a net negative portrayal.

Robot Chicken, a sketch comedy show, often employs shock humor and stereotypes. When transsexual characters or themes are present, they are typically used for comedic effect, often relying on mockery or problematic tropes. The portrayals generally lack dignity or complexity, contributing to a net negative impact without offering counterbalance or critique.

Robot Chicken frequently features Wonder Woman, who, despite her superpowers, is often depicted winning close-quarters physical fights against multiple male villains and henchmen using martial arts and Amazonian strength.

As a sketch comedy show, Robot Chicken frequently parodies established characters from pop culture and history. Given its comedic and subversive nature, it has, at various times, intentionally portrayed characters with a different gender than their canonical or historical baseline for comedic effect.

Robot Chicken consistently employs irreverent and often absurd humor to satirize Christian figures, biblical narratives, holidays, and cultural practices. The show frequently depicts religious elements in mundane or ridiculous contexts, highlighting perceived hypocrisies or subverting sacred imagery for comedic effect, without offering counterbalancing positive portrayals.

Robot Chicken occasionally features sketches that parody Jewish cultural elements or holidays, treating them with the same irreverent and absurd humor applied to other cultural and religious topics. These portrayals often play on stereotypes or specific traditions for comedic effect, aiming to make them appear silly or mundane, without providing any affirming counter-narrative.