Rowan & Martin's Laugh-In, a product of its era, included depictions of effeminate male characters and jokes playing on gender non-conformity. These portrayals often relied on stereotypes for comedic effect, presenting identity as a source of ridicule without offering dignity or complexity. The net impact is considered negative due to the use of problematic humor.

The show frequently employed satire and irreverent humor targeting Christian practices, institutions, and cultural stereotypes, often portraying adherents or clergy as foolish, hypocritical, or out of touch for comedic effect without offering counterbalancing positive portrayals.

Through its sketch comedy format, 'Laugh-In' included jokes and gags that sometimes relied on cultural stereotypes associated with Judaism, portraying adherents in ways that reinforced simplistic or negative views for comedic purposes without a narrative counterpoint affirming the faith's dignity.