RuPaul's Drag Race All Stars offers an overwhelmingly positive and affirming portrayal of LGBTQ+ characters and themes. It celebrates drag as an art form, showcasing the dignity, complexity, and agency of its queer contestants while fostering a powerful sense of community and self-acceptance.

RuPaul's Drag Race All Stars provides a significant platform for trans women to share their stories and artistry. The show consistently portrays trans characters with dignity and agency, celebrating their journeys of self-discovery and success within the drag community. Challenges faced by trans individuals are framed as external, reinforcing an affirming and empathetic stance towards trans identity.

The show frequently uses Christian iconography and themes in a satirical or subversive manner, often critiquing the perceived hypocrisy, judgment, or oppressive nature of religious institutions and conservative interpretations, particularly as they relate to LGBTQ+ identities. The narrative consistently champions self-acceptance and challenges traditional norms, often contrasting with rigid religious doctrines.