Senora Acero features Pepito, a prominent gay character whose identity and romantic relationship are explored with dignity and complexity. The show depicts the challenges of homophobia as external forces, maintaining an empathetic and affirming stance towards his queer identity and love.

Senora Acero features Rosario, a transsexual character whose journey includes navigating societal prejudice and gaining acceptance from her mother, La Tuti. The show portrays Rosario with dignity and agency, framing obstacles as external. La Tuti's arc from initial struggle to fierce support for her daughter contributes to a net positive and affirming portrayal of transsexual identity and the importance of familial acceptance.

The series portrays Christianity, particularly Catholicism, as an integral part of the characters' cultural and moral landscape. While characters may be deeply flawed or engage in criminal acts, the faith itself is often presented as a source of solace, moral guidance, or a framework for understanding sin and redemption, without being satirized or condemned by the narrative.