Spin City featured Carter Heywood, a prominent, openly gay main character. His portrayal was consistently positive, depicting him with dignity and complexity. His relationships were treated respectfully, affirming LGBTQ+ lives and love within a mainstream sitcom context.

Spin City featured a transsexual character in 'The Paul Affair,' where her identity was used as a comedic plot twist. The portrayal leaned heavily into negative stereotypes, presenting her transsexuality as a source of shock and discomfort for other characters, played for laughs without meaningful counter-critique.

The show often features Christian holidays and cultural references, treating them as a normal part of the characters' lives and the societal backdrop. While individual characters might be satirized for their personal flaws, the narrative does not portray Christianity itself as inherently negative or problematic, generally respecting its place in the culture.

The series includes a prominent Jewish character, Paul Lassiter, whose Jewish identity is part of his background and occasionally a source of comedic situations related to cultural references or personal neuroses. However, the portrayal is respectful of his heritage and does not depict Judaism as inherently negative or foolish, but rather as a valid aspect of a character's identity.