Superjail! incorporates LGBTQ+ coded characters and themes primarily for shock value and dark humor. Depictions often involve villainy, crude sexual acts, or humiliation, aligning with a problematic portrayal that lacks dignity or affirmation for queer identities within its chaotic and transgressive narrative.

Superjail! includes characters with ambiguous gender identities, such as Alice, and features numerous instances of gender non-conformity or body modification. These elements are consistently presented through the show's signature dark, grotesque, and often ridiculing humor, without any affirming or empathetic context. The overall portrayal aligns with problematic stereotypes, resulting in a net negative impact on transsexual themes.

The show features Alice, a physically imposing guard, who routinely engages in and wins close-quarters physical fights against multiple male prisoners, showcasing her strength and combat prowess.

The show consistently portrays Christianity through a lens of extreme satire, depicting its adherents and institutions as hypocritical, violent, or absurd. Religious iconography is frequently subverted for grotesque comedic effect, reinforcing a negative view of its manifestations within the Superjail universe.