The Teenage Mutant Ninja Turtles animated series, in its various iterations, does not explicitly feature LGBTQ+ characters or themes. The narratives consistently focus on the adventures of the four mutant turtles and their allies, without incorporating queer identities or storylines, resulting in no discernible LGBTQ+ portrayal.

The animated series "Teenage Mutant Ninja Turtles" (2012) does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of four mutant turtles and their rat sensei, with no elements related to transgender identity present in its storylines or character arcs.

The 2012–2017 Teenage Mutant Ninja Turtles series maintains the established genders for its core cast and recurring characters from previous iterations. No significant character established as one gender in prior canon is portrayed as a different gender in this adaptation.

The 2012-2017 "Teenage Mutant Ninja Turtles" series maintains the established races of its core human characters like April O'Neil and Casey Jones as white. While Baxter Stockman was white in the 1987 cartoon, his portrayal as Black in the 2012 series aligns with his original comic book depiction, thus not constituting a race swap from the primary source material.