The series 'The Boss Baby: Back in Business' does not include any discernible LGBTQ+ characters or themes. The narrative focuses on the adventures of Boss Baby and Tim in the corporate world of BabyCorp, without incorporating elements related to queer identity or experiences.

The animated series 'The Boss Baby: Back in Business' does not feature any identifiable transsexual characters or themes. Its narrative is entirely focused on the comedic adventures of a secret agent baby and his family, thus rendering the portrayal N/A.

The animated series primarily features comedic, slapstick action and relies on gadgets, schemes, and group efforts by the baby characters. While female characters participate in missions, there are no clear instances where a female character definitively defeats one or more male opponents in direct physical combat through skill, strength, or martial arts.

The animated series continues the story with the established characters from the original film, such as Boss Baby and Tim Templeton, all retaining their canonical genders. No previously established character undergoes a gender change.

The animated series continues the story of the Templeton family from the original film and book. All established characters maintain their original racial depictions, and no character canonically established as one race is portrayed as a different race.