The Boys features prominent LGBTQ+ characters, notably Queen Maeve, whose bisexuality is portrayed with dignity and complexity. Her challenges stem from external oppressive forces, not her identity, and her arc ultimately affirms the worth of her queer relationships and her fight for authenticity.

The show features female characters like Kimiko and Queen Maeve who demonstrate superior physical combat skills and strength against male opponents. Kimiko repeatedly defeats and kills multiple male adversaries in close-quarters fights, while Queen Maeve physically engages and neutralizes a powerful male supe.

The show features significant characters like Stormfront and Victoria Neuman, who were established as male in the original comic book series but are portrayed as female in the television adaptation.

Several key characters, including A-Train, Mother's Milk, and Black Noir, who were depicted as white in the original comic book series, are portrayed by Black actors in the television adaptation.

The show consistently portrays Christianity as a tool for corporate manipulation, political power, and extremist ideologies, particularly through Vought International's PR and characters like Homelander and Stormfront. It satirizes the hypocrisy and corruption of those who exploit faith for personal gain and control, rather than critiquing the faith itself.