The League includes LGBTQ+ characters and themes, primarily through Ruxin's gay brother, Geoffrey. However, the show frequently uses these elements for problematic humor, relying on stereotypes, the discomfort of straight characters, and employing 'gay' as a pejorative. The narrative often presents these views for laughs without significant critique, resulting in a net negative portrayal.

The League includes transsexual characters, notably in an episode where a character's trans identity is used as a source of problematic humor. The portrayal relies on the main cast's discomfort and mockery, reinforcing negative stereotypes without offering counterbalancing positive elements or critique.

The show frequently uses Jewish characters and cultural practices as a source of comedic conflict. While the humor is often directed at character flaws, the portrayal can reinforce comedic stereotypes of Jewish families and traditions without significant counterbalancing nuance.