The Love Boat rarely featured explicit LGBTQ+ themes. The most notable instance involved a comedic plot where two women were mistakenly identified as a lesbian couple, with humor stemming from the misunderstanding. The show's overall portrayal of LGBTQ+ themes was incidental and reflective of its era, avoiding strong positive or negative arcs.

The Love Boat featured an episode in 1980 with Pat, a transsexual woman, seeking acceptance from her son. While Pat is depicted with dignity and the story resolves with her son's acceptance, the narrative primarily frames her identity as a source of conflict for others to overcome. The portrayal avoids strong denigration but also lacks a deeply affirming stance, making its net impact neutral.

The show frequently features elements culturally associated with Christianity, such as weddings officiated by ministers and themes of forgiveness and moral redemption. These are consistently portrayed in a respectful and affirming light, aligning with positive social values without critiquing the faith itself.

When Jewish characters or cultural practices are depicted, they are presented with respect and as an integral, valued part of the diverse human experiences on board. The narrative avoids negative stereotypes and affirms the dignity of the faith and its adherents.