The animated series 'The Powerpuff Girls' does not explicitly feature any identifiable LGBTQ+ characters or themes. While some characters may exhibit gender-nonconforming traits, these are not presented as explicit LGBTQ+ identities within the show's narrative, leading to a determination of no direct depiction.

The Powerpuff Girls, an animated series, does not include any identifiable transsexual characters or themes. The show's narrative is centered on three superpowered girls fighting crime and does not explore gender identity in the context of transsexual experiences.

The Powerpuff Girls (1998–2004) is the original animated series. All characters, including the protagonists and villains, were created for this show with their established genders. There are no pre-existing characters from prior canon or history whose gender was altered for this production.

The Powerpuff Girls (1998–2004) is the original animated series where its characters were first established. There are no instances within this specific show where a character's race was changed from a previously established canonical depiction.