The animated series "The Real Adventures of Jonny Quest" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on action-adventure plots without incorporating queer identities or storylines.

The animated series "The Real Adventures of Jonny Quest" does not feature any identifiable transsexual characters or themes. Its narrative focuses on action, adventure, and science fiction elements, with no depiction of transgender identity.

The show features female characters such as Jessie Bannon and Jade, who are intelligent, resourceful, and participate in various adventures. However, there are no prominent or recurring instances where these characters are depicted as victorious in close-quarters physical combat against one or more male opponents through skill, strength, or martial arts.

The Real Adventures of Jonny Quest features the original core characters—Jonny, Hadji, Dr. Quest, and Race Bannon—all retaining their established male genders from the prior series. Jessie Bannon is a new character, not a gender swap.

The animated series maintains the established racial identities of its core characters from the original Jonny Quest canon. No characters canonically established as one race were portrayed as a different race in this adaptation.